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Polyclonal Antibody to Immunoglobulin G (IgG)

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[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Traits: Liquid
Concentration: 0.5mg/mL
UOM: 20µL
Cross Reactivity: N/A
Applications: WB; IHC; ICC; IP.
[ IMMUNOGEN ]
Immunogen: Native IgG
Accession No.: NPA544Mu01
[ APPLICATIONS ]
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50%  glycerol.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated  thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ IDENTIFICATION ]

     

Western Blot; Sample: Mouse Serum

Primary Ab: 0.2µg/ml Rabbit Anti Mouse
IgG Antibody Second Ab:
0.2µg/mL HRP-Linked Caprine Anti Rabbit
IgG Polyclonal Antibody
 
Western Blot; Samples: Lane1: Mouse
Serum; Lane2: Mouse Plasma;
Primary Ab: 0.02µg/ml Rabbit Anti Mouse IgG Antibody
Second Ab: 0.2μg/ml HRP-Linked
Caprine Anti-Rabbit IgG Polyclonal Antibody
 
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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