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人硫酸角质素(KS)酶联免疫吸附检测试剂盒

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Product Name：人硫酸角质素(KS)酶联免疫吸附检测试剂盒
Alternative Names：KS; Keratan Sulfate
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：31.9 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：2h
Research Area：Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KS in the samples is then determined by comparing the OD of the samples to the standard curve.

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