人全反钴胺素(Holo TC)酶联免疫吸附检测试剂盒
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Product Name：人全反钴胺素(Holo TC)酶联免疫吸附检测试剂盒
Alternative Names：Holo-TC
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：0.045 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma and other biological fluids
Assay length：2.5h
Research Area：Metabolic pathway;Nutrition metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：Holo-TC
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：0.045 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma and other biological fluids
Assay length：2.5h
Research Area：Metabolic pathway;Nutrition metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve.
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