小鼠抗胰岛素自身抗体(Anti-IAA)酶联免疫吸附检测试剂盒
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Product Name：小鼠抗胰岛素自身抗体(Anti-IAA)酶联免疫吸附检测试剂盒
Alternative Names：Anti-Insulin; Antiinsulin; Insulin Antibodies
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.32 ng/mL
standard：50 ng/mL
Detection range：0.78-50 ng/mL
Sample type：Serum, plasma and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;Endocrinology;Autoimmunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：Anti-Insulin; Antiinsulin; Insulin Antibodies
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.32 ng/mL
standard：50 ng/mL
Detection range：0.78-50 ng/mL
Sample type：Serum, plasma and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;Endocrinology;Autoimmunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.
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