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> 牛性别决定区Y框蛋白9(SOX9)酶联免疫吸附检测试剂盒
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牛性别决定区Y框蛋白9(SOX9)酶联免疫吸附检测试剂盒

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Product Name：牛性别决定区Y框蛋白9(SOX9)酶联免疫吸附检测试剂盒
Alternative Names：CMPD1; CMD1; SRA1; Campomelic Dysplasia,Autosomal Sex-Reversal; Transcription factor SOX-9
Species：Cattle
Assay Type：Sandwich
Sensitivity：0.121 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Reproductive science;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SOX9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SOX9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SOX9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SOX9 in the samples is then determined by comparing the OD of the samples to the standard curve.

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