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> 大鼠人AMP激活蛋白激酶α1(PRKAa1)酶联免疫吸附检测试剂盒
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大鼠人AMP激活蛋白激酶α1(PRKAa1)酶联免疫吸附检测试剂盒

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Product Name：大鼠人AMP激活蛋白激酶α1(PRKAa1)酶联免疫吸附检测试剂盒
Alternative Names：AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
Species：Rat
Assay Type：Sandwich
Sensitivity：0.14 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Enzyme & Kinase;Metabolic pathway;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRKAa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRKAa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRKAa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRKAa1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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