Product Name：兔核因子κB亚基p65(NFκB-p65)酶联免疫吸附检测试剂盒
Alternative Names：NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
Species：Rabbit
Assay Type：Sandwich
Sensitivity：17.5 pg/mL
standard：3000 pg/mL
Detection range：46.88-3000 pg/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Apoptosis;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

内容整理中...