人6酮前列腺素F1α(6-K-PGF1α)酶联免疫吸附检测试剂盒
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Alternative Names:6-keto-PGF1 alpha; 6ketoPGF1α
Species:Human
Assay Type:Competitive Inhibition
Sensitivity:5.4 pg/mL
standard:800 pg/mL
Detection range:12.5-800 pg/mL
Sample type:Serum, plasma and other biological fluids.
Assay length:2.5h
Research Area:Neuro science;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 6-K-PGF1α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 6-K-PGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 6-K-PGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.
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