小鼠消退素E1(RvE1)酶联免疫吸附检测试剂盒

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Product Name:小鼠消退素E1(RvE1)酶联免疫吸附检测试剂盒
Alternative Names:RvE1
Species:Mouse
Assay Type:Competitive Inhibition
Sensitivity:6.97 pg/mL
standard:1000 pg/mL
Detection range:15.63-1000 pg/mL
Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:2.5h
Research Area:Immunology & Inflammation
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse RvE1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RvE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RvE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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