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> 大鼠G蛋白偶联受体131(GPR131)酶联免疫吸附检测试剂盒
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大鼠G蛋白偶联受体131(GPR131)酶联免疫吸附检测试剂盒

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Product Name：大鼠G蛋白偶联受体131(GPR131)酶联免疫吸附检测试剂盒
Alternative Names：GPBAR1; GPCR; BG37; GPCR19; M-BAR; TGR5; G Protein-Coupled Bile Acid Receptor 1; G-protein coupled receptor GPCR19; Membrane-type receptor for bile acids
Species：Rat
Assay Type：Sandwich
Sensitivity：0.11 ng/mL
standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：Serum, plasma and other biological fluids.
Assay length：3.5h
Research Area：Neuro science;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GPR131. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GPR131. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GPR131, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GPR131 in the samples is then determined by comparing the OD of the samples to the standard curve.

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