唾液酸(SA)酶联免疫吸附检测试剂盒
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Product Name：唾液酸(SA)酶联免疫吸附检测试剂盒
Alternative Names：SA
Species：General
Assay Type：Competitive Inhibition
Sensitivity：25.5 µg/mL
standard：5000 µg/mL
Detection range：78.13-5000 µg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids.
Assay length：2.5h
Research Area：Signal transduction;Tumor immunity;Hepatology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：SA
Species：General
Assay Type：Competitive Inhibition
Sensitivity：25.5 µg/mL
standard：5000 µg/mL
Detection range：78.13-5000 µg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids.
Assay length：2.5h
Research Area：Signal transduction;Tumor immunity;Hepatology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve.
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