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> 大鼠生长分化因子10(GDF10)酶联免疫吸附检测试剂盒
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大鼠生长分化因子10(GDF10)酶联免疫吸附检测试剂盒

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Product Name：大鼠生长分化因子10(GDF10)酶联免疫吸附检测试剂盒
Alternative Names：BMP-3b, BMP3B,bone morphogenetic protein 3B,BIP,
Species：Rat
Assay Type：Sandwich
Sensitivity：0.12 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Signal Transduction;Cell Biology;Stem Cells;Cancer
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF10 in the samples is then determined by comparing the OD of the samples to the standard curve.

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