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> 人支链-氨基酸氨基转移酶,线粒体(BCAT2)酶联免疫吸附检测试剂盒
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人支链-氨基酸氨基转移酶,线粒体(BCAT2)酶联免疫吸附检测试剂盒

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Product Name：人支链-氨基酸氨基转移酶,线粒体(BCAT2)酶联免疫吸附检测试剂盒
Alternative Names：Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT(m); Bcatm; Eca40; Bcat2
Species：Human
Assay Type：Sandwich
Sensitivity：12.8 pg/mL
standard：3000 pg/mL
Detection range：46.88-3000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;Metabolic pathway
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BCAT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BCAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BCAT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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