犬β2微球蛋白(β2-MG)酶联免疫吸附检测试剂盒
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Product Name：犬β2微球蛋白(β2-MG)酶联免疫吸附检测试剂盒
Alternative Names：Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Species：Dog
Assay Type：Sandwich
Sensitivity：0.23 µg/mL
standard：50 µg/mL
Detection range：0.78-50 µg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：Infection immunity;Kidney biomarker;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Species：Dog
Assay Type：Sandwich
Sensitivity：0.23 µg/mL
standard：50 µg/mL
Detection range：0.78-50 µg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：Infection immunity;Kidney biomarker;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
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