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> 小鼠B-淋巴细胞激活抗原B7-2(LAB7-2)酶联免疫吸附检测试剂盒
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小鼠B-淋巴细胞激活抗原B7-2(LAB7-2)酶联免疫吸附检测试剂盒

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Product Name：小鼠B-淋巴细胞激活抗原B7-2(LAB7-2)酶联免疫吸附检测试剂盒
Alternative Names：CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.048 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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