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> 小鼠压电型机械敏感离子通道组件1(PIEZO-1)酶联免疫吸附检测试剂盒
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小鼠压电型机械敏感离子通道组件1(PIEZO-1)酶联免疫吸附检测试剂盒

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Product Name：小鼠压电型机械敏感离子通道组件1(PIEZO-1)酶联免疫吸附检测试剂盒
Alternative Names：PIEZO1; FAM38A; KIAA0233
Species：Mouse
Assay Type：Sandwich
Sensitivity：22.3 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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