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> 牛氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒
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牛氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒

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Product Name：牛氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒
Alternative Names：NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Species：Cattle
Assay Type：Sandwich
Sensitivity：12 pg/mL
standard：2500 pg/mL
Detection range：39.07-2500 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Endocrinology;Cardiovascular biology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

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