大鼠涎液化糖链抗原6(KL-6)酶联免疫吸附检测试剂盒

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Product Name:大鼠涎液化糖链抗原6(KL-6)酶联免疫吸附检测试剂盒
Alternative Names:KL-6
Species:Rat
Assay Type:Sandwich
Sensitivity:13.44 U/mL
standard:2000 U/mL
Detection range:31.25-2000 U/mL
Sample type:serum, plasma, tissue homogenates and other biological fluids
Assay length:3.5h
Research Area:Interstitial lung disease
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

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