牛心肌肌钙蛋白T(cTnT/TNNT2)酶联免疫吸附检测试剂盒
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Product Name：牛心肌肌钙蛋白T(cTnT/TNNT2)酶联免疫吸附检测试剂盒
Alternative Names：CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Species：Cattle
Assay Type：Sandwich
Sensitivity：26.1 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Cardiovascular biology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Species：Cattle
Assay Type：Sandwich
Sensitivity：26.1 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Cardiovascular biology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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