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> 大鼠胱天蛋白酶4(CASP4)酶联免疫吸附检测试剂盒
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大鼠胱天蛋白酶4(CASP4)酶联免疫吸附检测试剂盒

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Product Name：大鼠胱天蛋白酶4(CASP4)酶联免疫吸附检测试剂盒
Alternative Names：ICE(rel)II; ICH2; TX; Protease ICH-2; Cysteinyl Aspartate Specific Proteinases 4; Apoptosis-Related Cysteine Peptidase
Species：Rat
Assay Type：Sandwich
Sensitivity：1.13 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Enzyme & Kinase;Apoptosis;Infection immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CASP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CASP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CASP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CASP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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