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> 大鼠钙蛋白酶2(CAPN2)酶联免疫吸附检测试剂盒
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大鼠钙蛋白酶2(CAPN2)酶联免疫吸附检测试剂盒

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Product Name：大鼠钙蛋白酶2(CAPN2)酶联免疫吸附检测试剂盒
Alternative Names：CANPL2; CANPml; mCANP; Calpain-2 Catalytic Subunit; Calcium-activated neutral proteinase 2; Calpain M-type; Calpain large polypeptide L2; Millimolar-calpain
Species：Rat
Assay Type：Sandwich
Sensitivity：0.054 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;Enzyme & Kinase;Neuro science;Gastroenterology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CAPN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CAPN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CAPN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CAPN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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