人多个EGF样结构域8(MEGF8)酶联免疫吸附检测试剂盒
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Product Name：人多个EGF样结构域8(MEGF8)酶联免疫吸附检测试剂盒
Alternative Names：C19orf49; CRPT2; EGFL4; SBP1
Species：Human
Assay Type：Sandwich
Sensitivity：0.063 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, saliva, tears and other biological fluids and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEGF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEGF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEGF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEGF8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：C19orf49; CRPT2; EGFL4; SBP1
Species：Human
Assay Type：Sandwich
Sensitivity：0.063 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, saliva, tears and other biological fluids and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEGF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEGF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEGF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEGF8 in the samples is then determined by comparing the OD of the samples to the standard curve.
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