一步法人白介素2受体a链(IL-2Ra/CD25)酶联免疫吸附检测试剂盒

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Product Name:一步法人白介素2受体a链(IL-2Ra/CD25)酶联免疫吸附检测试剂盒
Alternative Names:IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Species:Human
Assay Type:Sandwich
Sensitivity:37.66 pg/mL
standard:6000 pg/mL
Detection range:93.75-6000 pg/mL
Sample type:Serum, plasma
Assay length:1.5h
Research Area:Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

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