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> 一步法大鼠25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒
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一步法大鼠25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒

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Product Name：一步法大鼠25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒
Alternative Names：25 Hydroxy Vitamin D;
Species：Rat
Assay Type：Competitive Inhibition
Sensitivity：7.7 ng/mL
standard：5000 ng/mL
Detection range：78.13-5000 ng/mL
Sample type：Serum, plasma
Assay length：1.5h
Research Area：Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

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