一步法人血管细胞粘附分子1(VCAM1)酶联免疫吸附检测试剂盒

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Product Name:一步法人血管细胞粘附分子1(VCAM1)酶联免疫吸附检测试剂盒
Alternative Names:CD106; INCAM-100; L1CAM
Species:Human
Assay Type:Sandwich
Sensitivity:0.87 ng/mL
standard:80 ng/mL
Detection range:1.25-80 ng/mL
Sample type:Serum, plasma
Assay length:1.5h
Research Area:CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1, and the Human VCAM1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VCAM1. After TMB substrate solution is added, only those wells that contain Human VCAM1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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