一步法人骨钙素(OC)酶联免疫吸附检测试剂盒
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Product Name:一步法人骨钙素(OC)酶联免疫吸附检测试剂盒
Alternative Names:BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Species:Human
Assay Type:Sandwich
Sensitivity:0.14 ng/mL
standard:15 ng/mL
Detection range:0.23-15 ng/mL
Sample type:Serum
Assay length:1.5h
Research Area:Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OC. After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.
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