一步法人白介素6(IL6)酶联免疫吸附检测试剂盒

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Product Name:一步法人白介素6(IL6)酶联免疫吸附检测试剂盒
Alternative Names:IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
Species:Human
Assay Type:Sandwich
Sensitivity:0.1 pg/mL
standard:100 pg/mL
Detection range:1.56-100 pg/mL
Sample type:serum, plasma
Assay length:1.5 h
Research Area:Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6, and the Human IL6 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL6. After TMB substrate solution is added, only those wells that contain Human IL6 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

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