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外观与性状:黑色粉末
包装规格:1g 5g 25g in glass bottle
溶解性:溶于水和1M氢氧化铵溶液
产品描述:基本信息 产品编号: R10009 产品名称: Resazurin sodium salt CAS: 62758-13-8   储存条件 粉末 室温 四年 分子式: C12H6NNaO4 溶于液体 -80℃(密封存放,防潮) 6个月 分子量: 251.17 -20℃(密封存放,防潮) 1个月 化学名:  Resazurin sodium salt,certified by the Biological Stain Commission Solubility (25°C):   体外:   DMSO   Ethanol   Water   体内(现配现用):   <1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 3.9814mL 19.9068mL 39.8137mL 5mM 0.7963mL 3.9814mL 7.9627mL 10mM 0.3981mL 1.9907mL 3.9814mL   生物活性 产品描述 氧化还原指示剂;厌氧指示剂。 靶点 Bacterial 体外研究 Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.No viable bacteria are detected 24h post-inoculation following inclusion of Resazurin sodium in TSBc cultures of F.tularensis LVS at the concentration of 44μM.Lowering the Resazurin sodium concentration to as little as 4.4μM still results in a 10-fold reduction in viable F.tularensis LVS compare to growth medium alone.Both Resazurin sodium treatments result in a significant decrease in viable F.tularensis LVS bacteria over 22h.Treatment with Resazurin sodium significantly reduces the number of viable F.tularensis LVS bacteria in HEK293 cells 22h post-infection.   推荐实验方法(仅供参考) 细胞实验:   F.tularensis is cultured in TSBc supplemented with 44μM Resazurin sodium salt at 37℃ with shaking for 24h.At select time points,a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600nm and 570nm to detect the presence of Resazurin sodium salt and resorufin,respectively.The ratio of these two optical densities is used to evaluate reduction of Resazurin sodium salt to resorufin over time.
保存条件:室温