β-Amyloid (35-42) ≥98%
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| 包装规格: | 5mg 10mg in glass bottle |
| 产品简介: | 是 β 淀粉样蛋白的多肽片段,由 35-42 位氨基酸序列组成。 |
| 溶解性: | 溶于DMSO(50mg/mL超声) |
| 储备液保存: | 该产品在溶液状态不稳定,建议您现用现配,即刻使用。 |
| 体内实验: | 1、请依序添加每种溶剂: 10% DMSO→90% (20% SBE-β-CD in Saline) Solubility: 2.5 mg/mL (3.36 mM); 悬浊液; 超声助溶 此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。 2、请依序添加每种溶剂: 10% DMSO→90% Corn Oil Solubility: ≥ 2.5 mg/mL (3.36 mM); 澄清溶液 此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液,此方案实验周期在半个月以上的动物实验酌情使用。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。 <1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
| 靶点: | Amyloid-β |
| 体外研究: | β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure. 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃. 3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing. 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments. Methods vary depends on the downstream applications |
| 保存条件: | -20℃ |
| 注意事项: | 1、为了您的安全和健康,请穿实验服并戴一次性手套操作。 2、以上信息仅做参考交流之用。 |
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