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包装规格：1mg 5mg 25mg 100mg in glass bottle
产品描述：基本信息 产品编号：R10461 产品名称：Rituximab CAS: 174722-31-7   储存条件 粉末 -20℃ 四年 分子式： C6416H9874N1688O1987S44 溶于液体 -80℃ 六个月 分子量       化学名：      Solubility (25°C)   体外 DMSO   Ethanol   Water   体内（现配现用）     ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   生物活性 产品描述 一种抗 CD20 嵌合单克隆抗体，用于某些自身免疫疾病和癌症的研究。 靶点/IC50 CD20   体外研究 Rituximab inhibits the proliferation of stimulated human B cells, which is associated with a relative increase of B cells with an activated naive phenotype. Aside from this population shift, there are no major changes in phenotype or cytokine profile of the various B-cell subsets. B cells stimulated in the presence of rituximab induces stronger T-cell proliferation, compared to B cells stimulated in the absence of rituximab. All lymphoma cells tested are equally sensitive to antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-mediated phagocytosis of tumor cells,and rituximab-induced apoptosis.Rituximab induces high CDC killing of follicular lymphoma cells. 体内研究 A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor, cures 100% of the animals.Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent.   推荐实验方法(仅供参考) 激酶实验： 生化激酶实验测定IC50和动力学研究 IC50测量使用poly-Glu–Tyr（4:1）或者poly-Lys–Tyr（4:1，针对Lck）为底物。二价阳离子为20mM MgCl2（对于Src、Fyn、Yes、Lyn、Csk、Frk或Abl）或10mM MnCl（对于FGFR1、IGF1R、Lck或Met）。ATP终浓度为：Src,10μM；Fyn,6μM；Yes,100μM；Lyn,2μM；Csk,10μM；Frk,10μM；Abl,4μM；FGFR1,10μM；IGF1R,2μM；Lck,2μM；Met,5μM；PDGFR,6μM。PDGFRb的磷酸化IC50值是通过测量免疫沉淀的PDGFRb确定的。Km值使用Eadie-Hofstee法计算。   细胞实验:   A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor,cures 100% of the animals.Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent.   动物实验:   C57BL/6 mice (8-10 wk of age) are inoculated 8×103 EL4-CD20+cells in 200μL of saline by tail vein injection. In parallel groups of mice,150g of rituximab, murine anti-CD20 IgG2a Ab 1F5, or control anti-human IL-2R Ab daclizumab in 300μL of saline, or saline only is inoculated i.p. 24h later.
保存条件：-20℃

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