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包装规格：1g 5g 25g in glass bottle
溶解性：溶于DMSO（≥ 35 mg/mL）
产品描述：基本信息 产品编号： C10917 产品名称： Carmustine CAS: 154-93-8   储存条件 粉末 -20℃ 四年     分子式： C5H9Cl2N3O2 溶于液体 -20℃ 两年 分子量 214.05     化学名：  N,N'-BIS(2-CHLOROETHYL)-N-NITROSOUREA Solubility (25°C)：   体外:   DMSO ≥ 35mg/mL (163.51mM) Ethanol   Water 100mg/mL (467.18mM; Need ultrasonic) 体内(现配现用): 1.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5mg/mL (11.68mM); Clear solution 此⽅案可获得 ≥ 2.5mg/mL (11.68mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。 2.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.08mg/mL (9.72mM); Clear solution 此⽅案可获得 ≥ 2.08mg/mL (9.72mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.08mg/mL (9.72mM); Clear solution 此⽅案可获得 ≥ 2.08mg/mL (9.72mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 4.6718mL 23.3590mL 46.7181mL 5mM 0.9344mL 4.6718mL 9.3436mL 10mM 0.4672mL 2.3359mL 4.6718mL   生物活性 产品描述 DNA 和 RNA 的烷化剂 (alkylator)。 靶点 DNA Alkylator 体外研究 Carmustine is an antitumor chemotherapeutic agent. Carmustine (8, 80, and 800µM) decreases N-acetyltransferase (NAT) activities for 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) in rat glial tumor cytosol and intact cells. In rat glial tumor cells, the DNA-AF adduct increases, and carmustine decreases the formation of DNA-AF adduct 体内研究 Carmustine (BCNU; 25mg/kg, i.p.) causes higher levels of the rhe ratio of liver weight to body weight and plasma conjugated bilirubin, and lower biliary flow, oxidised glutation levels (GSSG) and reduced glutation (GSH)/GSSG values compared with control rats   推荐实验方法(仅供参考) Kinase Assay The determination of Acetyl-CoAdependent N-acetylation of 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) are performed. Incubation mixtures in the assay system consists of a total volume of 90μL: glial tumor cells cytosols, diluted as required, in 50μL of lysis buffer (20mM Tris/HCl, pH 7.5, 1mM DTT and 1mM EDTA), 20μL of an Acetyl-CoA recycling mixture of 50mM Tris-HCl (pH7.5), 0.2mM EDTA, 2mM DTT, 15mM acetylcamitine, 2U/mL carnitine acetyltransferase, and AF or PABA at specific concentrations. The reactions are started by addition of 20μL of Acetyl-CoA. The control reactions have 20μL distilled water in place of Acetyl-CoA. For the single point activity measurements, the final concentration of AF or PABA is 0.1mM and AcCoA is 0.5mM. The reaction mixtures with or without specific concentrations of Carmustine and lomustine are incubated at 37°C for 10 min and stopped with 50μL of 20% trichloroacetic acid for the PABA reactions, and 100μL of acetonitrile for the AF reactions. All of the reactions (experiments and controls) are run in triplicate Animal Administration Rats Individual rats are weighted prior to enter the study; their weights are recorded, and they are randomLy assigned to four groups. Group I (saline group); This group consists of 12 rats. These rats are injected with 2mL/kg of saline intraperitoneally (IP) 48 h before the study, being included by the study 48 h later. Group II (corn oil group) consists of 15 rats. These rats are injected with 2mL/kg of corn oil (vehicle) IP 48 h before the study. Group III (Carmustine group) consists of 16 rats. These rats are injected with 1mL per day of saline IP, administered at the same hour of the day as a single-dose for 3 days. Twelve hours after the first dose of saline, corn oil 2mL/kg + Carmustine 25mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine. Group IV (trimetazidine group) consists of 12 rats. These rats are injected with 2.5mg/kg per day of trimetazidine (TMZ) IP, administered at the same hour of the day as a single-dose for 3 days. 12 h after the first dose of TMZ, corn oil 2mL/kg + Carmustine 25mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine
保存条件：-20℃

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