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> 小鼠酮戊二酸脱氢酶(OGDH)酶联免疫吸附检测试剂盒
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小鼠酮戊二酸脱氢酶(OGDH)酶联免疫吸附检测试剂盒

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Product Name：小鼠酮戊二酸脱氢酶(OGDH)酶联免疫吸附检测试剂盒
Alternative Names：AKGDH; E1k; OGDC; Alpha-Ketoglutarate Dehydrogenase,Lipoamide; 2-Oxoglutarate Dehydrogenase E1 Component,Mitochondrial
Species：Mouse
Assay Type：Sandwich
Sensitivity：1.15 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Enzyme & Kinase;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OGDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OGDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OGDH in the samples is then determined by comparing the OD of the samples to the standard curve.

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