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> 鸡二肽基肽酶Ⅳ(DPP4)酶联免疫吸附检测试剂盒
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鸡二肽基肽酶Ⅳ(DPP4)酶联免疫吸附检测试剂盒

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Product Name：鸡二肽基肽酶Ⅳ(DPP4)酶联免疫吸附检测试剂盒
Alternative Names：CD26; ADA; DPPIV; DPP-IV; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26
Species：Chicken
Assay Type：Sandwich
Sensitivity：0.266 ng/mL
standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Endocrinology;Cardiovascular biology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken DPP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken DPP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken DPP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken DPP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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