人C1q肿瘤坏死因子相关蛋白4(C1QTNF4)酶联免疫吸附检测试剂盒
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Product Name：人C1q肿瘤坏死因子相关蛋白4(C1QTNF4)酶联免疫吸附检测试剂盒
Alternative Names：C1QT4; C1QT4; Complement C1q tumor necrosis factor-related protein 4
Species：Human
Assay Type：Sandwich
Sensitivity：19.37 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：Serum, plasma, tissue homogenates
Assay length：3.5h
Research Area：Tumor immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1QTNF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1QTNF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1QTNF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：C1QT4; C1QT4; Complement C1q tumor necrosis factor-related protein 4
Species：Human
Assay Type：Sandwich
Sensitivity：19.37 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：Serum, plasma, tissue homogenates
Assay length：3.5h
Research Area：Tumor immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1QTNF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1QTNF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1QTNF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
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