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> 大鼠蛋白激酶Bγ(PKBg)酶联免疫吸附检测试剂盒
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大鼠蛋白激酶Bγ(PKBg)酶联免疫吸附检测试剂盒

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Product Name：大鼠蛋白激酶Bγ(PKBg)酶联免疫吸附检测试剂盒
Alternative Names：PKBG; PRKB-G; AKT3; RAC-PK-Gamma; RAC-Gamma; STK2; RAC-Gamma Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 3
Species：Rat
Assay Type：Sandwich
Sensitivity：23.6 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Enzyme & Kinase;Tumor immunity;Endocrinology;Neuro science;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKBg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKBg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKBg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKBg in the samples is then determined by comparing the OD of the samples to the standard curve.

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