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> 人β-骨胶原交联(bCTx)酶联免疫吸附检测试剂盒
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人β-骨胶原交联(bCTx)酶联免疫吸附检测试剂盒  

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Product Name：人β-骨胶原交联(bCTx)酶联免疫吸附检测试剂盒
Alternative Names：Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：36.16 pg/mL
standard：8000 pg/mL
Detection range：125-8000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：2h
Research Area：Bone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human bCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.

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