人凝血因子ⅩⅢB肽(F13B)酶联免疫吸附检测试剂盒
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Product Name：人凝血因子ⅩⅢB肽(F13B)酶联免疫吸附检测试剂盒
Alternative Names：F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：1.24 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：plasma
Assay length：2h
Research Area：Signal transduction;Metabolic pathway;Hematology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：1.24 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：plasma
Assay length：2h
Research Area：Signal transduction;Metabolic pathway;Hematology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve.
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