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人CD83分子(CD83)酶联免疫吸附检测试剂盒

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Product Name：人CD83分子(CD83)酶联免疫吸附检测试剂盒
Alternative Names：BL11; HB15; Activated B Lymphocytes,Immunoglobulin; B-cell activation protein; Cell surface protein HB15
Species：Human
Assay Type：Sandwich
Sensitivity：18.3 pg/mL
standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：Serum, plasma, cell lysates and other biological fluids.
Assay length：3.5h
Research Area：CD & Adhesion molecule;Infection immunity;Immune molecule;Dermatology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD83. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD83. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD83, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD83 in the samples is then determined by comparing the OD of the samples to the standard curve.

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