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> 鸡C反应蛋白(CRP)酶联免疫吸附检测试剂盒
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鸡C反应蛋白(CRP)酶联免疫吸附检测试剂盒

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Product Name：鸡C反应蛋白(CRP)酶联免疫吸附检测试剂盒
Alternative Names：C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Species：Chicken
Assay Type：Sandwich
Sensitivity：0.112 ng/mL
standard：500 ng/mL
Detection range：7.81-500 ng/mL
Sample type：serum, plasma and other biological fluids
Assay length：3.5h
Research Area：Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

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