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> 大鼠高敏C反应蛋白(hs-CRP)酶联免疫吸附检测试剂盒
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大鼠高敏C反应蛋白(hs-CRP)酶联免疫吸附检测试剂盒

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Product Name：大鼠高敏C反应蛋白(hs-CRP)酶联免疫吸附检测试剂盒
Alternative Names：C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Species：Rat
Assay Type：Sandwich
Sensitivity：0.16 µg/mL
standard：50 µg/mL
Detection range：0.78-50 µg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

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