人DNA酶Ⅰ样蛋白3(DNASE1L3)酶联免疫吸附检测试剂盒
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Product Name：人DNA酶Ⅰ样蛋白3(DNASE1L3)酶联免疫吸附检测试剂盒
Alternative Names：DHP2; DNAS1L3; LSD; Deoxyribonuclease Gamma; Liver and spleen DNase; DNase I homolog protein DHP2
Species：Human
Assay Type：Sandwich
Sensitivity：0.14 ng/mL
standard：50 ng/mL
Detection range：0.78-50 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids.
Assay length：3.5h
Research Area：Enzyme & Kinase;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNASE1L3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNASE1L3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNASE1L3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNASE1L3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：DHP2; DNAS1L3; LSD; Deoxyribonuclease Gamma; Liver and spleen DNase; DNase I homolog protein DHP2
Species：Human
Assay Type：Sandwich
Sensitivity：0.14 ng/mL
standard：50 ng/mL
Detection range：0.78-50 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids.
Assay length：3.5h
Research Area：Enzyme & Kinase;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNASE1L3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNASE1L3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNASE1L3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNASE1L3 in the samples is then determined by comparing the OD of the samples to the standard curve.
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