人抗β2-糖蛋白1抗体IgA酶联免疫吸附检测试剂盒

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Product Name:人抗β2-糖蛋白1抗体IgA酶联免疫吸附检测试剂盒
Alternative Names:APOH; BG; B2G1; B2GP1
Species:Human
Assay Type:Sandwich
Sensitivity:26.8 pg/mL
standard:4000 pg/mL
Detection range:62.5-4000 pg/mL
Sample type:Serum, plasma and other biological fluids.
Assay length:3.5h
Research Area:Immune molecule;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human β2-GP1 lgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human β2-GP1 lgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human β2-GP1 lgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human β2-GP1 lgA in the samples is then determined by comparing the OD of the samples to the standard curve.

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