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> 大鼠瞬时受体电位阳离子通道亚家族V成员4(TRPV4)酶联免疫吸附检测试剂盒
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大鼠瞬时受体电位阳离子通道亚家族V成员4(TRPV4)酶联免疫吸附检测试剂盒

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Product Name：大鼠瞬时受体电位阳离子通道亚家族V成员4(TRPV4)酶联免疫吸附检测试剂盒
Alternative Names：SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
Species：Rat
Assay Type：Sandwich
Sensitivity：18.35 pg/mL
standard：3000 pg/mL
Detection range：46.88-3000 pg/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRPV4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRPV4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRPV4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRPV4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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