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> 大鼠4-羟基壬烯酸(HNE) 酶联免疫吸附检测试剂盒
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大鼠4-羟基壬烯酸(HNE) 酶联免疫吸附检测试剂盒

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Product Name：大鼠4-羟基壬烯酸(HNE) 酶联免疫吸附检测试剂盒
Alternative Names：4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
Species：Rat
Assay Type：Sandwich
Sensitivity：0.67 ng/mL
standard：100 ng/mL
Detection range：1.57-100 ng/mL
Sample type：Serum, plasma, tissue homogenates
Assay length：3.5h
Research Area：Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

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