人丝状肌动蛋白(F-actin)酶联免疫吸附检测试剂盒
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Product Name：人丝状肌动蛋白(F-actin)酶联免疫吸附检测试剂盒
Alternative Names：Fibrous Actin
Species：Human
Assay Type：Sandwich
Sensitivity：0.18 ng/mL
standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：Serum, plasma, tissue homogenates
Assay length：3.5h
Research Area：Signal transduction;Developmental science;Bone metabolism;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：Fibrous Actin
Species：Human
Assay Type：Sandwich
Sensitivity：0.18 ng/mL
standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：Serum, plasma, tissue homogenates
Assay length：3.5h
Research Area：Signal transduction;Developmental science;Bone metabolism;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.
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