人细胞间粘附分子2(ICAM2)酶联免疫吸附检测试剂盒
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Product Name：人细胞间粘附分子2(ICAM2)酶联免疫吸附检测试剂盒
Alternative Names：CD102
Species：Human
Assay Type：Sandwich
Sensitivity：0.12 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Serum, plasma and other biological fluids.
Assay length：3.5h
Research Area：CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ICAM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ICAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ICAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ICAM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：CD102
Species：Human
Assay Type：Sandwich
Sensitivity：0.12 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Serum, plasma and other biological fluids.
Assay length：3.5h
Research Area：CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ICAM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ICAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ICAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ICAM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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