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> 马硫代巴比妥酸反应物(TBARS)酶联免疫吸附检测试剂盒
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马硫代巴比妥酸反应物(TBARS)酶联免疫吸附检测试剂盒

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Product Name：马硫代巴比妥酸反应物(TBARS)酶联免疫吸附检测试剂盒
Alternative Names：TBARS; Thiobarbituric Acid Reactive Substance
Species：Horse
Assay Type：Competitive Inhibition
Sensitivity：3.55 nmol/mL
standard：400 nmol/mL
Detection range：6.25-400 nmol/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length：2h
Research Area：Metabolic pathway;Hepatology;Hormone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse TBARS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.

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