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> 一步法人胰岛素样生长因子结合蛋白1(IGFBP1)酶联免疫吸附检测试剂盒
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一步法人胰岛素样生长因子结合蛋白1(IGFBP1)酶联免疫吸附检测试剂盒

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Product Name：一步法人胰岛素样生长因子结合蛋白1(IGFBP1)酶联免疫吸附检测试剂盒
Alternative Names：IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein
Species：Human
Assay Type：Sandwich
Sensitivity：0.54 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：Serum, plasma
Assay length：1.5h
Research Area：Tumor immunity;Endocrinology;Cardiovascular biology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP1, and the Human IGFBP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP1. After TMB substrate solution is added, only those wells that contain Human IGFBP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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