一步法人表皮生长因子受体2(EGFR2)酶联免疫吸附检测试剂盒
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Alternative Names:CD340; ErbB2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
Species:Human
Assay Type:Sandwich
Sensitivity:16.9 pg/mL
standard:5000 pg/mL
Detection range:78.13-5000 pg/mL
Sample type:Serum, plasma
Assay length:1.5h
Research Area:Signal transduction;Tumor immunity;Reproductive science;Hepatology;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2, and the Human EGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR2. After TMB substrate solution is added, only those wells that contain Human EGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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