一步法人单核细胞趋化蛋白1(MCP1)酶联免疫吸附检测试剂盒

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Product Name:一步法人单核细胞趋化蛋白1(MCP1)酶联免疫吸附检测试剂盒
Alternative Names:CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE
Species:Human
Assay Type:Sandwich
Sensitivity:0.57 pg/mL
standard:500 pg/mL
Detection range:7.81-500 pg/mL
Sample type:Serum, plasma, cell culture supernatants
Assay length:1.5h
Research Area:Cytokine;Tumor immunity;Infection immunity;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP1, and the Human MCP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MCP1. After TMB substrate solution is added, only those wells that contain Human MCP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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